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Ocean Ridge Biosciences microarray
(A) <t>Microarray</t> analysis of miRNA regulation in liver samples from diseased rainbow trout infected with Viral hemorrhagic septicaemia virus (VHSV). The Ncode V2 probe set covering miRBase v.9 was used to detect up-(blue) and down-regulated (red) miRNAs. The miRNA probes are denoted according to the miRBase nomenclature. Organisms: dre = Danio rerio , hsa = Homo sapiens and dme = Drosophila melanogaster . (B) qPCR validation of miR-462 and miR-731 expression in the liver of VHSV-infected rainbow trout. Fold regulation was calculated from the mean values of duplicate measurements using the ΔΔC t method, using the omy-snoRNA U23 for normalization. miRNA expression was analyzed in 6 VHSV-infected fish relative to 6 uninfected fish.
Microarray, supplied by Ocean Ridge Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) <t>Microarray</t> analysis of miRNA regulation in liver samples from diseased rainbow trout infected with Viral hemorrhagic septicaemia virus (VHSV). The Ncode V2 probe set covering miRBase v.9 was used to detect up-(blue) and down-regulated (red) miRNAs. The miRNA probes are denoted according to the miRBase nomenclature. Organisms: dre = Danio rerio , hsa = Homo sapiens and dme = Drosophila melanogaster . (B) qPCR validation of miR-462 and miR-731 expression in the liver of VHSV-infected rainbow trout. Fold regulation was calculated from the mean values of duplicate measurements using the ΔΔC t method, using the omy-snoRNA U23 for normalization. miRNA expression was analyzed in 6 VHSV-infected fish relative to 6 uninfected fish.
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(A) <t>Microarray</t> analysis of miRNA regulation in liver samples from diseased rainbow trout infected with Viral hemorrhagic septicaemia virus (VHSV). The Ncode V2 probe set covering miRBase v.9 was used to detect up-(blue) and down-regulated (red) miRNAs. The miRNA probes are denoted according to the miRBase nomenclature. Organisms: dre = Danio rerio , hsa = Homo sapiens and dme = Drosophila melanogaster . (B) qPCR validation of miR-462 and miR-731 expression in the liver of VHSV-infected rainbow trout. Fold regulation was calculated from the mean values of duplicate measurements using the ΔΔC t method, using the omy-snoRNA U23 for normalization. miRNA expression was analyzed in 6 VHSV-infected fish relative to 6 uninfected fish.
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Ocean Ridge Biosciences multiplex protein profiling
(A) <t>Microarray</t> analysis of miRNA regulation in liver samples from diseased rainbow trout infected with Viral hemorrhagic septicaemia virus (VHSV). The Ncode V2 probe set covering miRBase v.9 was used to detect up-(blue) and down-regulated (red) miRNAs. The miRNA probes are denoted according to the miRBase nomenclature. Organisms: dre = Danio rerio , hsa = Homo sapiens and dme = Drosophila melanogaster . (B) qPCR validation of miR-462 and miR-731 expression in the liver of VHSV-infected rainbow trout. Fold regulation was calculated from the mean values of duplicate measurements using the ΔΔC t method, using the omy-snoRNA U23 for normalization. miRNA expression was analyzed in 6 VHSV-infected fish relative to 6 uninfected fish.
Multiplex Protein Profiling, supplied by Ocean Ridge Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) <t>Microarray</t> analysis of miRNA regulation in liver samples from diseased rainbow trout infected with Viral hemorrhagic septicaemia virus (VHSV). The Ncode V2 probe set covering miRBase v.9 was used to detect up-(blue) and down-regulated (red) miRNAs. The miRNA probes are denoted according to the miRBase nomenclature. Organisms: dre = Danio rerio , hsa = Homo sapiens and dme = Drosophila melanogaster . (B) qPCR validation of miR-462 and miR-731 expression in the liver of VHSV-infected rainbow trout. Fold regulation was calculated from the mean values of duplicate measurements using the ΔΔC t method, using the omy-snoRNA U23 for normalization. miRNA expression was analyzed in 6 VHSV-infected fish relative to 6 uninfected fish.
Digital Oscilloscope Lecroy, supplied by LeCroy Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) <t>Microarray</t> analysis of miRNA regulation in liver samples from diseased rainbow trout infected with Viral hemorrhagic septicaemia virus (VHSV). The Ncode V2 probe set covering miRBase v.9 was used to detect up-(blue) and down-regulated (red) miRNAs. The miRNA probes are denoted according to the miRBase nomenclature. Organisms: dre = Danio rerio , hsa = Homo sapiens and dme = Drosophila melanogaster . (B) qPCR validation of miR-462 and miR-731 expression in the liver of VHSV-infected rainbow trout. Fold regulation was calculated from the mean values of duplicate measurements using the ΔΔC t method, using the omy-snoRNA U23 for normalization. miRNA expression was analyzed in 6 VHSV-infected fish relative to 6 uninfected fish.
Qms Density Profiler Qdp 01 X, supplied by Quintek Measurement Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) <t>Microarray</t> analysis of miRNA regulation in liver samples from diseased rainbow trout infected with Viral hemorrhagic septicaemia virus (VHSV). The Ncode V2 probe set covering miRBase v.9 was used to detect up-(blue) and down-regulated (red) miRNAs. The miRNA probes are denoted according to the miRBase nomenclature. Organisms: dre = Danio rerio , hsa = Homo sapiens and dme = Drosophila melanogaster . (B) qPCR validation of miR-462 and miR-731 expression in the liver of VHSV-infected rainbow trout. Fold regulation was calculated from the mean values of duplicate measurements using the ΔΔC t method, using the omy-snoRNA U23 for normalization. miRNA expression was analyzed in 6 VHSV-infected fish relative to 6 uninfected fish.
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Ocean Ridge Biosciences mirna array
(A) <t>Microarray</t> analysis of miRNA regulation in liver samples from diseased rainbow trout infected with Viral hemorrhagic septicaemia virus (VHSV). The Ncode V2 probe set covering miRBase v.9 was used to detect up-(blue) and down-regulated (red) miRNAs. The miRNA probes are denoted according to the miRBase nomenclature. Organisms: dre = Danio rerio , hsa = Homo sapiens and dme = Drosophila melanogaster . (B) qPCR validation of miR-462 and miR-731 expression in the liver of VHSV-infected rainbow trout. Fold regulation was calculated from the mean values of duplicate measurements using the ΔΔC t method, using the omy-snoRNA U23 for normalization. miRNA expression was analyzed in 6 VHSV-infected fish relative to 6 uninfected fish.
Mirna Array, supplied by Ocean Ridge Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Quintek Measurement Systems x-ray density profiler qdp-01x
(A) <t>Microarray</t> analysis of miRNA regulation in liver samples from diseased rainbow trout infected with Viral hemorrhagic septicaemia virus (VHSV). The Ncode V2 probe set covering miRBase v.9 was used to detect up-(blue) and down-regulated (red) miRNAs. The miRNA probes are denoted according to the miRBase nomenclature. Organisms: dre = Danio rerio , hsa = Homo sapiens and dme = Drosophila melanogaster . (B) qPCR validation of miR-462 and miR-731 expression in the liver of VHSV-infected rainbow trout. Fold regulation was calculated from the mean values of duplicate measurements using the ΔΔC t method, using the omy-snoRNA U23 for normalization. miRNA expression was analyzed in 6 VHSV-infected fish relative to 6 uninfected fish.
X Ray Density Profiler Qdp 01x, supplied by Quintek Measurement Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Microarray analysis of miRNA regulation in liver samples from diseased rainbow trout infected with Viral hemorrhagic septicaemia virus (VHSV). The Ncode V2 probe set covering miRBase v.9 was used to detect up-(blue) and down-regulated (red) miRNAs. The miRNA probes are denoted according to the miRBase nomenclature. Organisms: dre = Danio rerio , hsa = Homo sapiens and dme = Drosophila melanogaster . (B) qPCR validation of miR-462 and miR-731 expression in the liver of VHSV-infected rainbow trout. Fold regulation was calculated from the mean values of duplicate measurements using the ΔΔC t method, using the omy-snoRNA U23 for normalization. miRNA expression was analyzed in 6 VHSV-infected fish relative to 6 uninfected fish.

Journal: PLoS ONE

Article Title: Two Virus-Induced MicroRNAs Known Only from Teleost Fishes Are Orthologues of MicroRNAs Involved in Cell Cycle Control in Humans

doi: 10.1371/journal.pone.0132434

Figure Lengend Snippet: (A) Microarray analysis of miRNA regulation in liver samples from diseased rainbow trout infected with Viral hemorrhagic septicaemia virus (VHSV). The Ncode V2 probe set covering miRBase v.9 was used to detect up-(blue) and down-regulated (red) miRNAs. The miRNA probes are denoted according to the miRBase nomenclature. Organisms: dre = Danio rerio , hsa = Homo sapiens and dme = Drosophila melanogaster . (B) qPCR validation of miR-462 and miR-731 expression in the liver of VHSV-infected rainbow trout. Fold regulation was calculated from the mean values of duplicate measurements using the ΔΔC t method, using the omy-snoRNA U23 for normalization. miRNA expression was analyzed in 6 VHSV-infected fish relative to 6 uninfected fish.

Article Snippet: The microarray for detecting miRNA levels was run by a microarray profiling service (Ocean Ridge Biosciences, USA).

Techniques: Microarray, Infection, Virus, Biomarker Discovery, Expressing